EXPLORE INTERACTIONS
Discuss your project
RNA AND RBP BIOLOGY
Change the condition. Capture RNA–protein interactions. Explore two complementary measurements.
START HERE
Select a state, then capture the interactions below.
Changes in post-transcriptional regulation, including splicing, 3′-end processing, nuclear export, localization, translation, stability & decay, and epitranscriptional regulation, can be associated with altered RNA-interaction levels of RBPs and protein occupancy on RNA.
COMPOUND A
Each condition illustrates a steady-state interaction pattern across a population of ribonucleoprotein complexes.
The light pulse represents UV capture. Background clearance represents subsequent purification. Retained contacts are illustrative.
Compare RNA-interaction levels of proteins and protein occupancy on RNA following each treatment.
FROM CAPTURED INTERACTIONS TO MEASUREMENTS
RNA-bound protein profiling
Which proteins show altered association with RNA?
Start from the captured RNA–protein complexes.
Example proteins · illustrative units · total protein pool held equal.
RNA PROFILING
Which RNA regions show altered protein occupancy?
Start from the same captured RNA–protein complexes.
Occupancy accounts for RNA abundance; RNA abundance is held equal in this illustration.
Schematic profiling paths based on the captured example proteins and RNA regions. These comparisons illustrate the two measurements; they are not experimental results.